Protein Structure Determination by Molecular Replacement using High-Accuracy Protein Structure Modeling
نویسندگان
چکیده
منابع مشابه
Protein structure determination by MicroED.
In this review we discuss the current advances relating to structure determination from protein microcrystals with special emphasis on the newly developed method called MicroED. This method uses a transmission electron cryo-microscope to collect electron diffraction data from extremely small 3-dimensional (3D) crystals. MicroED has been used to solve the 3D structure of the model protein lysozy...
متن کاملMolecular fragment replacement approach to protein structure determination by chemical shift and dipolar homology database mining.
A novel approach is described for determining backbone structures of proteins that is based on finding fragments in the protein data bank (PDB). For each fragment in the target protein, usually chosen to be 7-10 residues in length, PDB fragments are selected that best fit to experimentally determined one-bond heteronuclear dipolar couplings and that show agreement between chemical shifts predic...
متن کاملpxGrid - High-Throughput Protein Structure Determination using Computational Grids
Structural biology research places significant demands upon high-performance computing. The elucidation of protein structures at atomic resolution is computationally demanding and requires user-friendly interfaces to high-performance computing resources. Fortunately, critical calculations are embarrassingly parallel and thus ideally suited to distributed computing. Here we discuss how we are us...
متن کاملHigh-resolution protein structure determination by serial femtosecond crystallography.
Structure determination of proteins and other macromolecules has historically required the growth of high-quality crystals sufficiently large to diffract x-rays efficiently while withstanding radiation damage. We applied serial femtosecond crystallography (SFX) using an x-ray free-electron laser (XFEL) to obtain high-resolution structural information from microcrystals (less than 1 micrometer b...
متن کاملFemtosecond Crystallography High - Resolution Protein Structure Determination by Serial
clicking here. colleagues, clients, or customers by , you can order high-quality copies for your If you wish to distribute this article to others here. following the guidelines can be obtained by Permission to republish or repurpose articles or portions of articles ): November 5, 2012 www.sciencemag.org (this information is current as of The following resources related to this article are ava...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2010
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2009.12.2507